Environmental Studies Journal
Volume 1, No. 1, 2022
Pages 36-45
DOI: 10.36108/esj/2202.10.0130
Antimicrobial effect of Sodium hypochlorite and Aloe vera (Aloe barbadensis miller) under varying conditions on coliform isolated from Oil polluted water from Apapa, Lagos
IKHIDE, Evbonmai Charles1, * OLU, Joshua2, YUSUF, Hajara Oyiza3, BELLO, Zainab3, ONYEKWULE, Millicent Chekwube3
1Centre for Environmental Studies and Sustainable Development, Lagos State University, Ojo
2Faculty of Environmental Science, Nasarawa State University, Keffi
3National Biotechnology Development Agency, Environmental Biotechnology and Bio conservation Department, Along Musa Yar’adua Expressway, Airport Road, Lugbe, Abuja
*Corresponding Author- Email: olu@lujosh.com Orcid No: http://orcid,org/0000-0002-5865-3640
Abstract
This study aims at evaluating the antimicrobial effect of Aloe vera and Sodium hypochlorite under varying parameters on coliform isolated from oil polluted water. Presumptive test was carried out on the oil polluted water sample obtained from Ibafon in Apapa, Lagos, Nigeria to determine the presence of coliforms; confirmatory test was done on the positive samples to determine the coliform thermo-tolerant characteristics. A Completed test was carried out to isolate Escherichia coli O157:H7. The oil polluted water shows a coliform count of >1800 cfu/100ml. Thermo-tolerant E. coli (TE), pathogenic E. coli (PE) and non-pathogenic E. coli (NE) were detected and isolated in the water sample. The efficacy of NaOCl and Aloe vera gel as antimicrobials on the isolates were recorded at 5% concentration. NaOCl at 0 and 25°C, pH 10 and 10hr sunlight exposure was active against the isolates. Aloe vera at 25°C, pH 4.0, and zero sunlight exposure time exhibited inhibitory effect on the growth of thermo-tolerant E. coli. Pathogenic E. coli isolate was resistant to aloe vera at all used pH. Aloe vera at 0 and 55°C, pH 6, and 10hr of sunlight exposure time inhibited non-pathogenic E. coli growth. Both Aloe vera and Sodium hypochlorite showed antimicrobial activities towards the coliform isolated in this study and their efficacy were dependent on the conditions at which they were utilized.
Keywords: Aloe vera, Coliform, Escherichia coli, oil polluted water, Sodium hypochlorite
